| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Godsoe, Eugene D (27)
|
NCUN-0 |
23.43 |
23.34 |
-0.09 |
| 2 |
Legard, Yusuke (19)
|
NCDUKE |
23.80 |
23.36 |
-0.44 |
| 3 |
King, Mckay D (23)
|
UTHAST |
23.64 |
24.10 |
0.46 |
| 3 |
Soric, Lovre (20)
|
CCCSUB |
23.98 |
24.10 |
0.12 |
| 5 |
Osgood, Lucas P (21)
|
OHWSU |
23.70 |
24.16 |
0.46 |
| 6 |
Poltash, Alex T (21)
|
CAUN-0 |
20.21 |
24.18 |
3.97 |
| 7 |
Manion, William G (22)
|
VAW&M |
25.19 |
24.20 |
-0.99 |
| 8 |
Timonera, Bryce P (20)
|
CCCSUB |
24.01 |
24.23 |
0.22 |
| 9 |
Stover, Mitchell (20)
|
OHWSU |
23.70 |
24.26 |
0.56 |
| 10 |
Kwiecien-Delaney, Bryce J (19)
|
MIMICH |
23.94 |
24.36 |
0.42 |
| 11 |
Bohman, Bryce A (25)
|
AZFORD |
|
24.58 |
- |
| 12 |
Valente, Alexander D (18)
|
CAUN-2 |
24.01 |
24.59 |
0.58 |
| 13 |
Wynter, Timothy S (19)
|
CAUN-0 |
24.00 |
24.98 |
0.98 |
| 14 |
Marciano, Vincent T (14)
|
NJMCSC |
24.10 |
25.08 |
0.98 |
| 15 |
Block, Jason R (25)
|
USUC |
28.00 |
28.62 |
0.62 |
| 16 |
Fletcher, Julian R (25)
|
CATROJ |
28.50 |
29.90 |
1.40 |
| 17 |
Altman, Ridge W (22)
|
CAUN-1 |
29.01 |
30.01 |
1.00 |